FastQCFastQC Report
Mon 27 Feb 2023
SRR3128647.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128647.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2360284
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196630.8330777143767445No Hit
GCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG37390.15841314011364732No Hit
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG36830.15604054427348574No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC35300.14955827349590134No Hit
CTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC32620.13820370768941365No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT26460.11210515344763596No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT66900.082.088211
CGTAGGG6550.078.237712
TAGGGTA8700.070.76484
TAGACGG3500.069.915151
AGAGGGC32450.069.661953
AGGGTAA11400.069.2579655
AGTAGGG36200.069.092992
TAGGGCA11200.068.816264
ATAGGGC12750.068.559563
CGAAGGG20250.068.490112
AGGGCAT21050.068.317925
GAATAGG13350.067.326831
TATAGGG18300.067.3102342
AGTTAGG6250.066.2580641
AGGGATG31650.066.225685
TAAGGGA19900.065.889533
TAGAGGG41350.065.831532
GGAGCTA40500.065.3308949
AGGGACT30700.065.213355
AGAAGGG80700.064.899682
Produced by FastQC (version 0.11.5)