FastQCFastQC Report
Mon 27 Feb 2023
SRR3128646.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128646.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2245490
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182470.8126066025678138No Hit
GCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG43480.1936325701739932No Hit
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG40810.18174206965962886No Hit
CTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC34950.15564531572173557No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC31690.14112732632966524No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCAGTTGTGG28470.12678747177676142No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT23640.10527768994740569No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA22970.10229393139136671No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT63150.081.3879851
CGTAGGG6550.073.8957062
TAGGGTA8250.073.478294
GAATAGG13600.072.955331
TAGGGCA9750.072.7772454
TATAGGG15800.071.380152
GGTAAGG13000.070.896731
ACGGGAT7250.070.001774
GTAGGGC9550.069.872813
CCGTACA14050.069.568123
AGTAGGG32250.069.067212
ATAGGGA17700.069.027693
TAAGGGA16300.068.613983
AGGGAAT24400.068.565045
GGAGCTA47350.068.481269
AGGGATG26450.068.40345
ATAGGGC11200.068.390043
AGTACGG3550.067.5546651
AGTTAGG5850.067.52071
AGAGGGC30000.067.5117343