FastQCFastQC Report
Mon 27 Feb 2023
SRR3128645.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128645.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2516234
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227600.9045263675794859No Hit
GCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG48490.1927086272580372No Hit
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG46890.18634991817136243No Hit
CTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC39590.1573383079634088No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC37520.14911172808252332No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT27550.10948902208618118No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT75800.083.305121
CGTAGGG6650.079.168612
TAGGGTA9300.075.29784
AGGGATG30150.070.92565
ATAGGGC14550.070.739193
AGTAGGG41500.070.452912
TAGGGCA11650.070.194374
GTAGGGT11300.069.873063
GGAGCTA51400.069.856889
AGTAAGG17450.069.838151
ATAGGGA21850.069.260153
TATAGGG20450.069.187842
GTAGGGA18450.068.77753
GGTAAGG14050.068.6540151
GAATAGG16400.068.571311
TAGGGAT14350.068.449984
AGTTAGG6050.068.4408951
AAGGGTA16700.067.541974
AGAGGGC33150.067.4842453
AGGGAAT26200.067.447445
Produced by FastQC (version 0.11.5)