FastQCFastQC Report
Mon 27 Feb 2023
SRR3128644.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128644.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3453349
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT294470.8527084867472126No Hit
GCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG60570.1753949571850398No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC53920.15613828778962105No Hit
CCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG53860.15596454340409846No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC37060.10731611545777735No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT94300.081.6008761
CGTAGGG6750.075.926962
TAGGGCA16800.073.571944
AGTAGGG53450.071.872
AGGGATG51200.071.32095
ATAGGGC22050.071.187483
GTAGGGA25600.070.8619463
ACGGGTA4050.070.784894
ATAGGGA31200.070.645423
TAGCGGG18100.070.3986052
ATAGCGG7700.070.290371
TAGGGAT25150.069.5137564
AGGGAAT45000.068.82385
GAATAGG22250.068.745151
AAGGGTA25850.068.722194
TAGGGTA14300.068.6872254
CGAAGGG37050.068.40282
TAAGGGA29100.068.153043
TAGACGG4100.067.726231
AATAGGG49450.067.5095752
Produced by FastQC (version 0.11.5)