FastQCFastQC Report
Mon 27 Feb 2023
SRR3128643.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128643.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3201415
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270540.8450638233406166No Hit
GCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG68160.21290585569193624No Hit
CCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG59280.1851681209715079No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC58990.18426227152680924No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCTTTACCT37240.11632356317440881No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC33460.10451628420557785No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT83950.081.000791
TAGGGCA17850.075.297024
TAGCGGG15750.073.399132
AGTAGGG47700.072.4111252
ATAGGGC20950.071.106883
CCGTACA20350.070.6632163
CGTAGGG7600.070.490022
TAGGGTA13450.069.880774
GAATAGG22500.069.808081
TAAGGGT11800.069.693533
AGGGAAT40050.069.468745
AGGGATG45300.069.3024445
CGTACAC20750.069.076714
TAGAGGG54350.068.912052
ATAGGGA31750.068.528883
AAGGGTA21450.068.1374
ATAGGGT14100.067.990373
GTAGGGT13550.067.62883
AATAGGG47850.067.5682752
AGTACGG4600.067.472661
Produced by FastQC (version 0.11.5)