FastQCFastQC Report
Mon 27 Feb 2023
SRR3128642.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128642.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3581287
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT335080.9356412931999027No Hit
GCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG71330.19917420748462775No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC68760.19199801635557273No Hit
CCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG68490.19124409744318174No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC39820.1111890781163308No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT99250.081.3671341
TAGGGCA18250.074.939354
ACGGGTA4100.073.362814
AGTAGGG54500.072.793472
CGGGTAT4850.071.708365
AGGGATG52350.070.4746255
GAATAGG21700.070.04951
TAAGGGA31200.069.74393
AGGGAAT43650.069.124275
TAAGGGT13900.068.975553
GTAGGGA26600.068.9069063
ATAGGGA33050.068.826183
TAGAGGG61100.068.623072
ATAGGGC22850.068.491683
CGAAGGG34000.068.295892
TAGCGGG21100.067.945992
AATAGGG51650.067.891442
GTACGGG9800.067.630022
CGTAGGG8550.067.6216052
AGGGTAA19550.067.311785
Produced by FastQC (version 0.11.5)