FastQCFastQC Report
Mon 27 Feb 2023
SRR3128641.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128641.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences822801
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81290.9879667137011259No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCAGGGAAGC54920.6674760968934165No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC53050.6447488517879779No Hit
CCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTG40100.48735964103106344Illumina PCR Primer Index 8 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC35830.4354637391058105Illumina PCR Primer Index 8 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTG32260.39207536208633675Illumina PCR Primer Index 8 (95% over 22bp)
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA22960.2790468169095565No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATG18320.2226540803912489No Hit
CGCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCT12990.15787535503724473Illumina PCR Primer Index 8 (95% over 21bp)
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCAGGGAAGCTC12340.14997551048187835No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCAGG11610.14110337736585152No Hit
ACTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTG10620.12907130642767814Illumina PCR Primer Index 8 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCT10470.12724826537643977Illumina PCR Primer Index 8 (95% over 21bp)
AAAGCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTT8490.10318412350009297No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT38100.086.9788741
ATAGCGG1400.084.05781
GCGGGAT3050.083.204514
AGGGATG18500.081.034865
AGGGAAT18000.080.674995
CGGGTCG353.86608E-880.5630955
CGCTACG9850.080.1541443
GCGTAGG1000.080.023031
CGTAGGG2250.079.408172
GCTACGA10100.078.635434
TCCGCTA10200.078.453961
AGTACGG600.078.453951
GCACCGA600.078.325238
GTAGGGT2350.077.991933
TACGACC10300.077.56486
AGGGTAT5350.077.3004155
CTACGAC10400.076.818985
TAGGGTA2400.076.36714
AGGGCAT7550.075.316715
GTCGAAT255.561291E-575.192229
Produced by FastQC (version 0.11.5)