FastQCFastQC Report
Mon 27 Feb 2023
SRR3128640.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128640.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences813027
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75850.9329333466170251No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCAGGGAAGC74820.9202646406576904No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC49850.6131407690027515No Hit
CCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTG45290.5570540707750173Illumina PCR Primer Index 8 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC40440.4974004553354317Illumina PCR Primer Index 8 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTG36090.443896697157659Illumina PCR Primer Index 8 (95% over 22bp)
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA29430.3619805984303104No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATG24970.3071238716549389No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCAGGGAAGCTC16900.2078651754492778No Hit
CGCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCT14770.18166678351395463Illumina PCR Primer Index 8 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTG12830.15780533733812038Illumina PCR Primer Index 8 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCT12830.15780533733812038Illumina PCR Primer Index 8 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCAGG11550.14206170274787921No Hit
AAAGCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTT9880.12152117949342396No Hit
ATCCGTACACCCACCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGT9740.11979921946011632No Hit
AGAGCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTT9370.11524832508637475No Hit
ATCCGTACACCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGC8900.10946745926027057No Hit
GGCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCT8470.10417858201511143Illumina PCR Primer Index 8 (95% over 21bp)
TAGACCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCT8450.1039325877246389No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGAT156.9029356E-493.995022
TCCGCTA8900.089.798141
GCTACGA8850.088.690024
CGCTACG8950.087.693683
CGTTTTT38550.085.731291
CTACGAC9350.084.455125
TACGACC9400.084.000726
CATACGG452.7284841E-1183.5768361
CGACCAA9350.080.9311758
ACGGGTC353.8629878E-880.5721054
AGGGATG18650.080.397055
ACGACCA9500.080.1480567
CGTAGGG2000.079.895762
AATGCGG1300.079.558711
AGGGTAC3500.079.2341165
ACGGGTA1250.078.960674
GCGGGAT3400.078.794794
AGGGAAT17400.077.7985465
GGATAGG5200.077.750561
CGGGTAT1150.077.657615
Produced by FastQC (version 0.11.5)