FastQCFastQC Report
Mon 27 Feb 2023
SRR3128639.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128639.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences898961
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94031.0459853097075402No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC57430.6388486263586518No Hit
CCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTG50960.5668766498212937Illumina PCR Primer Index 8 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC44470.49468219422199633Illumina PCR Primer Index 8 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTG40160.4467379563740807Illumina PCR Primer Index 8 (95% over 22bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCAGGGAAGC39890.4437344890379004No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA17930.19945247902856741No Hit
CGCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCT16250.18076423782566764Illumina PCR Primer Index 8 (95% over 21bp)
ATCCGTACACCCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATG13780.15328807367616615No Hit
AGCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCT13440.14950592962319834Illumina PCR Primer Index 8 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCAGG12420.1381594974642949No Hit
ACTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTG11140.12392083750018076Illumina PCR Primer Index 8 (95% over 22bp)
AAAGCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTT10270.11424299830582194No Hit
TAGACCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCT9920.11034961472188448No Hit
AGAGCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTT9790.10890350081927914No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCAGGGAAGCTC8990.10000433834170781No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCTA11900.087.3600541
CGCTACG11800.087.225413
CGTTTTT43000.086.750561
GCTACGA12150.084.325934
CTACGAC12200.083.980335
TACGACC12300.082.533366
TAGGGCG1550.081.867774
GTTACGG750.081.536051
TTACGGG1500.081.472552
ATAGCGG2100.080.6400451
AGGGTAC4550.080.568285
AGGGAAT19500.080.25845
AGGGATG21900.080.0471045
CGGGCGA650.079.5353555
CGGGTAT1200.078.330275
ACGACCA12550.078.267867
AAGGGTA5600.078.050524
CGACCAA12600.077.9572758
TAGGGTA2600.077.727734
AGGGCTC3950.077.338755
Produced by FastQC (version 0.11.5)