FastQCFastQC Report
Mon 27 Feb 2023
SRR3128637.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128637.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2346309
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189900.8093563124038649No Hit
GCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTG50850.21672337275269368Illumina PCR Primer Index 5 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTG44600.1900857900643095Illumina PCR Primer Index 5 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC40420.1722705747623182Illumina PCR Primer Index 5 (95% over 23bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGCATGATT27400.11677916250587624No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC27170.11579889946294372No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA24800.10569792810750842No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT64550.081.589311
GTAGGGC10250.072.438093
ACGGGTA2800.072.1694644
TAGGGCA13450.070.927844
TAAGGGA19450.070.5499953
AGATAGG11300.070.326621
ATAGGGC14800.069.854563
AGGGATG34750.069.782615
ATAACGG1900.069.297231
ATAGCGG5100.069.151651
AAGGGTA14550.068.795434
CCGTACA14350.068.770333
AGAGGGC34200.068.56593
TAGAGGG40300.068.448952
AAGAGGG74550.068.330622
CGTAGGG5350.067.634882
AGTAGGG34850.067.556742
GTAGGGA15950.067.4696663
CGTACAC14600.067.272334
AGGGCAT21450.067.261015
Produced by FastQC (version 0.11.5)