FastQCFastQC Report
Mon 27 Feb 2023
SRR3128636.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128636.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2625090
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234650.8938741147922548No Hit
GCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTG54920.2092118746404885Illumina PCR Primer Index 5 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTG49130.18715548800231613Illumina PCR Primer Index 5 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC48060.18307943727643622Illumina PCR Primer Index 5 (95% over 23bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC31120.11854831643867449No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT78500.081.932471
ATAGCGG6200.075.886671
AGGGATG40850.072.597695
TAGAGGG45350.072.356812
ATAGGGC16200.071.368333
TAGCGGG13050.070.967092
AGATAGG14150.069.493961
AAGGGCA37900.068.699884
GGTAAGG14650.068.4067841
TAAGGGA21950.068.303283
AGAGGGC38800.068.075373
AAGGGAT44600.067.7581564
CGAAGGG26550.066.577062
CGTAGGG6300.066.4126362
TATAGGG21150.066.237992
ATAAGGG33100.066.1849442
AGGGAAT32200.066.119235
ATAGGGA25400.066.057173
AAGAGGG86350.065.603352
GAGGGAT35650.064.9939354
Produced by FastQC (version 0.11.5)