FastQCFastQC Report
Mon 27 Feb 2023
SRR3128635.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128635.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2729839
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228140.8357269421383459No Hit
CTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGC57730.21147767322541733No Hit
GCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG57370.21015891413376392No Hit
CCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG50650.185542077756234No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCTAGGAAT41270.15118107697926508No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT29230.10707591180285722No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT75900.081.976871
AGTAGGG41050.070.088422
CGTAGGG7300.069.551882
ATAGGGC14300.069.341833
GTAGGGC12350.068.49433
CCGTACA19450.068.377893
TAGGGCA13850.068.201694
GTAGGGA21450.068.136843
CGAAGGG26650.067.7394942
GGGCGAT11250.067.6723566
AGTAAGG17450.067.159211
ATAGCGG5350.066.859241
TGTAGGG17750.066.743682
TCCGTAC20500.066.7339862
ACGGGAT6850.066.547234
AGGGCAT24250.065.308095
ATAAGGG34650.065.124742
CGTACAC20500.065.104844
GAATAGG15000.064.950231
AAGGGCG11250.064.7482454
Produced by FastQC (version 0.11.5)