FastQCFastQC Report
Mon 27 Feb 2023
SRR3128634.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128634.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2564947
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207660.8096073720041779No Hit
CTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGC66640.25981043662890496No Hit
GCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG63500.24756846827634255No Hit
CCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG54960.21427343333020135No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCTAGGAAT51640.2013296960911863No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA30020.11703945539615439No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT26000.10136661693204577No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT71900.082.011341
CGTAGGG6100.072.418452
ATAGGGC13350.070.05243
AGGGCAT20750.069.7604455
AGTAGGG38400.069.7581252
TAGGGTA7750.069.7344364
TAGGGAT13400.069.44034
AAGGGTA17850.069.241864
GTAGGGA18200.069.2013553
GGTAAGG16650.068.9097751
TCCGTAC23600.068.700242
CCGTACA22850.068.487173
ATAAGGG31650.068.450782
TGTAGGG16650.068.304972
CGTACAC22950.067.983984
CGAAGGG21650.067.941892
TATAGGG16950.067.373292
AGTAAGG13800.067.126261
TAGACGG4350.067.020511
ATAGCGG4500.066.876371
Produced by FastQC (version 0.11.5)