FastQCFastQC Report
Mon 27 Feb 2023
SRR3128633.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128633.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2862871
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260630.9103798250078331No Hit
CTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGC76570.26745878525438277No Hit
GCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG70990.24796786163260587No Hit
CCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG65460.22865158786407072No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT30720.10730486983171789No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT85950.081.24731
CGTAGGG8650.072.818052
TAACGGG9600.071.977452
TAGGGCA14100.070.997414
CGAAGGG28050.070.717992
GAATAGG15700.070.43511
GGATAGG15200.069.96581
GTAGGGA21450.069.456683
AGTAGGG40100.069.394922
GTAGGGC12500.069.181483
ACGGGAT7800.067.484724
AGGGAAT33100.067.444675
TACGGGT2300.067.432323
ATAAGGG34400.066.272542
TAGACGG4200.066.103381
GGTAAGG16900.065.990671
TAGGGAC13250.065.974964
AAGGGCA42900.065.950984
TAGGGCG5000.065.797614
TAAGGGA22100.065.712543
Produced by FastQC (version 0.11.5)