FastQCFastQC Report
Mon 27 Feb 2023
SRR3128632.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128632.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences690377
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79031.1447368611642625No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC52310.7577019512527213No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCAACCCATG30560.44265669337188235No Hit
CCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG27570.39934702343791867RNA PCR Primer, Index 16 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG25330.36690098308605296RNA PCR Primer, Index 14 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGC24050.3483603885992726RNA PCR Primer, Index 16 (95% over 23bp)
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA11310.1638235340980363No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC10930.15831929510977336No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCAAC10750.15571202401006987No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATG10210.14789021071095937No Hit
CGCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCT9500.13760597470657338RNA PCR Primer, Index 16 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCT8280.11993447058636078RNA PCR Primer, Index 16 (95% over 21bp)
AGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTA7610.11022962815968668No Hit
ACTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG7490.10849144742655101RNA PCR Primer, Index 14 (95% over 22bp)
AAAGCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTT7370.10675326669341535No Hit
TAGACCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCT7080.10255266325500416No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGTA254.922149E-793.990228
CGTAGGG1550.087.970932
CGTTTTT30700.087.419391
ATCGCGG1150.085.9790651
AGGGATG18400.084.7955255
GACCGAT500.084.59128
TAAGGGA8950.084.01363
TAGACGG452.7284841E-1183.704491
CTAACGG452.7284841E-1183.704491
ACGGGTA850.082.932554
GGGCGAT2600.081.33776
GGAATGC11000.081.173387
AGGGAAT15450.080.9106755
AAGGGAT18900.080.811694
CGAGGGA5300.080.689723
CGCTACG9500.080.633723
TCCGCTA9600.080.4347841
GGATGGC13000.080.253187
GCTACGA9500.080.139044
GAGGGAT13100.078.923094
Produced by FastQC (version 0.11.5)