FastQCFastQC Report
Mon 27 Feb 2023
SRR3128631.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128631.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences664149
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70991.0688866504353693No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC50690.7632323469582879No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCAACCCATG41100.6188370380742875No Hit
CCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG32020.48212072893281477RNA PCR Primer, Index 16 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG28690.4319813776727813RNA PCR Primer, Index 14 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGC27390.41240745676045587RNA PCR Primer, Index 16 (95% over 23bp)
ATCCGTACACCCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATG15060.2267563453381696No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA12940.19483579738883897No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCAAC10640.16020501423626327No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC10560.15900046525704323No Hit
ACTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG9750.14680440684244048RNA PCR Primer, Index 14 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCT9410.14168507368075536RNA PCR Primer, Index 16 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCT9110.13716801500868028RNA PCR Primer, Index 16 (95% over 21bp)
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCAACCCATGTC8620.12979015251095763No Hit
AAAGCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTT8150.12271342725803998No Hit
TAGACCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCT8110.12211115276842997No Hit
GGCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCT7800.11744352547395238RNA PCR Primer, Index 16 (95% over 21bp)
AGAGCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTT7260.10931281986421722No Hit
GAACCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTT7170.10795770226259468No Hit
AGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTA7020.10569917292655714No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCGG1150.089.970771
CGTTTTT28000.087.341761
CGCTACG9050.085.16823
CTAACGG500.084.654321
ATAGCGG1450.084.329971
GCTACGA9150.084.23744
GCGATAA452.7284841E-1183.546268
AGGGATG15150.083.449145
TAAGGGA6600.083.315183
AGGGAAT13350.083.0831455
CACGGGT850.082.938193
GGATGGC10000.082.71087
TCCGCTA9400.082.552961
TACGGGT401.035005E-982.247043
CTACGAC9550.080.709135
AAGGGTA4200.080.568534
CGAAGGG4800.080.288772
ACGGGTA1300.079.535594
GGAATGC9300.078.8299267
GGTAAGG3050.078.6406251
Produced by FastQC (version 0.11.5)