FastQCFastQC Report
Mon 27 Feb 2023
SRR3128630.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128630.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences736790
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89591.2159502707691472No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC58090.7884200382741352No Hit
CCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG36650.4974280324108633RNA PCR Primer, Index 16 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG30900.41938679949510715RNA PCR Primer, Index 14 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGC29870.4054072395119369RNA PCR Primer, Index 16 (95% over 23bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCAACCCATG21480.29153490139659877No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCAAC12190.16544741378140312No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC11440.15526812253152186No Hit
AGCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCT10350.14047421924836115RNA PCR Primer, Index 16 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCT10030.13613105498174516RNA PCR Primer, Index 16 (95% over 21bp)
AAAGCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTT9580.1300234802318164No Hit
TAGACCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCT9060.1229658382985654No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA8860.12025136063193041No Hit
AGAGCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTT8560.1161796441319779No Hit
ACTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG8500.1153653008319874RNA PCR Primer, Index 14 (95% over 22bp)
GGCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCT7400.10043567366549491RNA PCR Primer, Index 16 (95% over 21bp)
GAACCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTT7400.10043567366549491No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGCG1300.090.3819354
CGTTTTT36200.088.6466141
ATAGCGG800.088.224181
CGCTACG9600.085.1849753
AGGGATG18500.085.105595
CGTAGGG2000.084.620462
GGATGGC12700.084.375457
TAAGGGA9000.083.0308763
CTACGAC9800.082.9669345
AGGGAAT14550.082.368695
CTAACGG800.082.342571
GCTACGA9950.082.188524
GAGGGAT14900.081.064724
TCCGCTA10250.080.793261
ATGGCAC11250.080.628729
GCGATAA353.8635335E-880.5690468
CGACCAA9750.080.500188
ACGACCA9800.079.6098867
GTAGGGC2450.078.6507343
AAGGGAT17950.078.54924
Produced by FastQC (version 0.11.5)