FastQCFastQC Report
Mon 27 Feb 2023
SRR3128629.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128629.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2197388
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183670.8358560254265519No Hit
GCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG34620.15755069200341498No Hit
CCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG30840.14034845006889998No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT29940.13625267817972975No Hit
CTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC29250.13311258639803258No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT62350.080.9084241
CGTAGGG6450.078.726992
GTAGGGA19100.070.864853
TAGGGCA11650.070.596624
AGTAAGG13350.070.49921
TAGGGCG3800.070.4957664
ATAGGGC12000.069.712493
AGTAGGG34750.069.680622
TAAGGGA19400.069.284493
AGGGAAT23900.068.23445
ATAGGGA20100.067.806883
CGAAGGG23000.067.45992
TATAGGG16950.067.1282352
TAGTAGG12650.066.21631
AGGGATG30450.066.2127765
TAGCGGG11400.065.577052
GAGGGAT27650.065.439114
AAGGGAC21500.065.3588644
AAGGGAT38500.064.941554
AGTACGG3050.064.8014761
Produced by FastQC (version 0.11.5)