FastQCFastQC Report
Mon 27 Feb 2023
SRR3128628.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128628.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2081254
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167860.806532984441111No Hit
GCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG39250.18858822613674256No Hit
CCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG34890.16763931744996047No Hit
CTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC34150.16408376872789193No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT27430.13179554249505346No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCTCCAGGT23080.11089468176397499No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT57750.080.7706761
CGTAGGG6850.075.466832
ATAGGGC10800.073.9740143
GGTAAGG12050.071.409851
AGGGATG26950.071.3246155
TAGGGCG3900.069.890374
AGTAGGG36000.068.665292
GTAGGGA18400.068.449613
ATGCGGG11800.068.103292
AATGCGG4350.068.0997241
GAATAGG13400.067.724621
AGGGAAT24400.067.607085
TAAGGGA20300.067.599093
TAGGGCA10900.067.259274
CGAAGGG20700.067.200942
ATAGGGA17500.067.136083
ATAAGGG28650.066.433082
ACTAGGG9200.066.406342
CCGTACA14550.066.213253
TATAGGG13850.066.16662
Produced by FastQC (version 0.11.5)