FastQCFastQC Report
Mon 27 Feb 2023
SRR3128627.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128627.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2341914
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209610.895037136291085No Hit
GCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG44040.18805131187567092No Hit
CCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG39830.17007456294296033No Hit
CTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC34880.14893800540925073No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT30170.1288262506650543No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT66800.083.337631
CGTAGGG7500.075.210692
GTAGGGC11850.071.388843
GAATAGG14950.069.249131
AGGGAAT29550.069.0253145
CGAAGGG24350.068.91742
GGTAAGG13400.068.831011
AGTAGGG38200.068.1722
ATAGGGA22900.067.931113
TAGGGCG4200.067.13954
TAAGGGA22850.067.051353
ATAAGGG33450.067.031962
TAGGGAC14750.066.593284
ATAGGGC12500.066.5486763
TATAGGG16950.066.003492
TAGCGGG13500.065.809362
TAGGGCA10400.065.525574
GTAGGGA22200.065.203953
TAGTAGG12750.064.9584051
AGTAAGG13350.064.858881
Produced by FastQC (version 0.11.5)