FastQCFastQC Report
Mon 27 Feb 2023
SRR3128626.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128626.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1284031
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129321.007140793329756No Hit
GCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG33840.26354503902164356TruSeq Adapter, Index 14 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG30490.23745532623433546TruSeq Adapter, Index 14 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC27670.2154932396491985Illumina PCR Primer Index 3 (95% over 23bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCATTGCGTC17080.1330185953454395No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT14990.11674172975574576No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC14590.11362654016920151No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA12970.1010100223436973No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT42450.082.2554861
GTAGGGC5750.075.196953
CGTAGGG2950.074.91042
TAGGGCA7550.074.698954
ACGGGTA1400.073.854144
GTAGGGA8750.071.4370963
AGTAGGG18650.070.842482
ATAGGGC8400.070.497143
CGAAGGG11250.070.213892
AGGGAAT16400.070.210575
ACGGGAT4850.069.770364
TAGGGAT8700.069.686824
AGATAGG6300.069.46741
TAAGGGA13050.069.1466143
AGGGCAT10300.068.9001165
TAGCGGG5750.068.687512
ACTAGGG5850.068.317092
AGGGATG17850.068.1933065
AGTAAGG8700.068.153781
AAGAGGG39150.068.095372
Produced by FastQC (version 0.11.5)