FastQCFastQC Report
Mon 27 Feb 2023
SRR3128625.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128625.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1245370
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118020.9476701703108313No Hit
GCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG37630.3021591976681629TruSeq Adapter, Index 14 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG34780.27927443249797246TruSeq Adapter, Index 14 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC31360.251812714293744Illumina PCR Primer Index 3 (95% over 23bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCATTGCGTC23870.19166994547805072No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA14610.11731453303034439No Hit
AGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCT12560.10085356159213728Illumina PCR Primer Index 3 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT37350.084.242241
CGTAGGG3350.075.755112
CGATTTA255.5639866E-575.187938
AGGGAAT14850.074.687615
TAGGGCA7350.072.8921054
TAGGGAT8000.072.25674
CCGTACA10400.070.946223
GCGTAGG2000.070.5481641
CGTACAC10500.070.2705464
AATAGGG16300.069.773542
AGGGATG17250.069.472695
ATCCGTA11050.069.377681
TCCGTAC11100.069.0124742
CGAGGGA8250.068.9277953
AGTAGGG18200.068.686792
GAGGGAT16100.068.305084
GTATAGG4000.068.1965561
AGTACGG2000.068.1965561
AGGGTAC5250.068.032635
AAGGGTC7400.067.9544
Produced by FastQC (version 0.11.5)