FastQCFastQC Report
Mon 27 Feb 2023
SRR3128624.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128624.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1381572
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149541.0823902047812202No Hit
GCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG39890.28872907094237577TruSeq Adapter, Index 14 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG38380.27779949217268446TruSeq Adapter, Index 14 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC35160.25449270830619036Illumina PCR Primer Index 3 (95% over 23bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC15500.11219104035113624No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT14110.10213003737771177No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT43700.082.8114241
ACGGGAT5300.075.374114
AGTAGGG20600.073.245312
ATAGCGG2400.072.5497361
TAGGGTA5600.072.175464
AGGGATG18450.071.3248445
ATAGGGA13650.071.271653
GAGGGAT15350.071.032774
TAAGGGA12250.070.976533
AGGGAAT17500.070.631245
AGGGTAC6300.069.377965
TAGGGAT9700.069.285674
GTAGGGC6450.069.221823
TAGGGCG3200.069.0282754
CGTAGGG3700.068.601572
CGAAGGG13900.068.3091052
AGTAAGG7800.067.572311
AAGAGGG44050.067.225912
AAGGGAT22250.066.958914
AGGGCGA8150.066.892825
Produced by FastQC (version 0.11.5)