FastQCFastQC Report
Mon 27 Feb 2023
SRR3128623.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128623.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1648721
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172211.044506620586503No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC38390.23284715849437232No Hit
GCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG36630.22217221713073343No Hit
CCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG34870.2114972757670946No Hit
CTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC31320.18996543381202763No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGTACTCGG25640.15551448668392046No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT51900.084.798111
CGTAGGG3300.082.660872
TAAGGGA16250.077.217273
AGGGATG29900.075.7586755
AAGGGTA12550.074.144344
ATAGGGA15900.073.8923343
TAGGGCA6550.073.183874
AAGGGCG6250.072.1851354
AGTAGGG25200.072.0398252
AGGGCAT12150.071.943775
AGGTACG200.002163963970.4932865
GGTAAGG8650.070.196511
AGGGAAT21550.070.002625
GGGCGAT5800.069.683026
AGGGTAT8850.069.5645
AAGGGAT29950.069.512594
GGTACGC750.068.926777
TAGCGGG7600.068.071462
AGGGTAA7400.067.9529955
ATAGAGG10900.067.797721
Produced by FastQC (version 0.11.5)