FastQCFastQC Report
Mon 27 Feb 2023
SRR3128622.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128622.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1574624
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155990.9906491962525656No Hit
GCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG42510.26996921169752275No Hit
CCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG38310.24329617737313797No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC37860.24043835226695387No Hit
CTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC34870.22144969211697524No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGTACTCGG32840.20855772552685592No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA16850.1070096734204483No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT49200.084.220061
TAGGGCA6450.076.5097964
CGTAGGG3400.076.030012
TAAGGGA15250.075.508783
ATAGCGG2850.074.2629851
GGTAAGG8200.073.9912951
AGGGATG26800.073.654955
CTACGAC8000.072.848255
AGTAGGG26400.071.92482
TAGACGG1450.071.3607561
ATAGGGA15550.071.029493
TATAAGG3600.070.5498351
GAATAGG9100.070.291411
CGCTACG8300.070.2174153
TACGACC8300.070.212956
ATAGGGC7300.070.178633
AGGGAAT21200.069.833235
TCCGCTA8550.069.312121
CGAAGGG13650.069.209332
AAGGGTA10650.068.4021154
Produced by FastQC (version 0.11.5)