FastQCFastQC Report
Mon 27 Feb 2023
SRR3128621.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128621.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1767846
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195691.1069403104116535No Hit
GCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG45940.25986426419495817No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC43140.2440257805261318No Hit
CCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG43090.24374295046061703No Hit
CTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC37630.21285790730640564No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGTACTCGG18130.10255418175565068No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT58950.084.5138241
ACGGGTA1550.078.836464
TAAGGGA15600.078.331093
CGTAGGG4100.075.664932
TAGGGCA6500.074.474794
AGTAGGG28400.073.9816062
GGTAAGG10150.073.6964651
AGGGATG31700.072.4995045
AGGGAAT24150.072.0062265
AGGGCAT15650.071.173685
ATAAGGG20600.070.049612
GGGCGAT5250.069.826586
CGAAGGG15300.069.737922
ACGGGAT6150.069.542734
ATAGCGG3600.069.261031
ATAGGGA20350.069.054543
TAGAGGG27900.068.905472
AAGGGAT31250.068.881234
AGGGACT21650.068.815595
CTACGAC8000.068.7355355
Produced by FastQC (version 0.11.5)