FastQCFastQC Report
Mon 27 Feb 2023
SRR3128620.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128620.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2934527
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT267010.9098910999966946No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT93200.083.231331
ATAGGGC16700.074.292393
GAATAGG20300.071.184911
TAGGGCA18200.070.7515644
GTAGGGC14600.070.1714553
AGGGATG43250.069.8685465
AGTAGGG47600.068.560792
ATAGGGA31450.067.6915363
AAGGGAT49150.067.6009454
CGAAGGG27350.067.2268452
TATAGGG21650.066.681262
TAGAGGG55250.066.38742
AGGGCAT26500.066.325815
AAGGGTA22050.065.64464
ACGGGTA4100.065.335294
AATAGGG44650.065.191732
CGTAGGG8950.065.151132
ATAGCGG6550.064.676541
AGTAAGG21150.064.095531
AGACGGG21550.063.717522
Produced by FastQC (version 0.11.5)