FastQCFastQC Report
Mon 27 Feb 2023
SRR3128619.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128619.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2745459
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246700.898574700988068No Hit
GCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG29930.10901637941051023No Hit
CCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG29530.1075594281320537No Hit
CTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC28620.10424486397356508No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT83050.083.6737751
ATAGCGG5750.073.591771
GTACGGG9200.072.533972
TAGGGCA16150.071.8742454
ATAGGGC16250.071.430643
AGTAGGG44700.070.5433352
ATAGGGA27700.070.0666353
AGGGATG38550.069.97655
AGTACGG4800.068.566411
AGTAAGG19050.067.625451
GTAGGGC13350.067.5866553
CCGTACA9750.067.478343
TAGGGAT19400.067.342924
TAGAGGG48050.066.309742
CGTAGGG6050.066.024412
TATAGGG21100.065.7022862
ACGGGAT8600.065.5740054
ATGTAGG10050.065.496271
GGGCGAT13850.065.4872066
TAGCGGG16150.065.180312
Produced by FastQC (version 0.11.5)