FastQCFastQC Report
Mon 27 Feb 2023
SRR3128618.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128618.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3104719
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT304790.9816991489406932No Hit
CCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG33480.10783584601376163No Hit
GCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG32730.10542016845968992No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT106950.083.590571
ATAGGGC19400.074.857853
ACGGGTA4350.074.548764
GTACGGG7550.072.220682
TAGGGCA18150.071.986124
TAGAGGG52600.069.972372
ATAGCGG6300.069.458741
AGTAGGG49850.069.400592
GAATAGG18600.068.555271
TATAGGG24500.068.11032
AGGGATG46150.067.823965
ATAGGGA33500.067.761483
AAGGGAT54000.067.0158544
GTAGGGC15600.066.580693
CGTAGGG8650.066.297062
CGAAGGG26900.065.877882
AGAGGGC42100.065.306193
AGTAAGG22100.065.149881
TAAGGGA27850.065.1392443
TAGGGTA9550.064.960764
Produced by FastQC (version 0.11.5)