FastQCFastQC Report
Mon 27 Feb 2023
SRR3128617.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128617.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences700305
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69870.9977081414526529No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGGAGTTAC44850.6404352389316084No Hit
CCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTG32740.46751058467382073No Hit
GCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTG26090.37255195950335923No Hit
CTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC25030.35741569744611273TruSeq Adapter, Index 27 (95% over 22bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC18770.2680260743533175No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA18260.26074353317483095No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATG15320.21876182520473222No Hit
CGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCT10810.1543613139989005No Hit
ACTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTG9590.1369403331405602TruSeq Adapter, Index 27 (95% over 21bp)
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCGGAGTTACTC9480.1353695889648082No Hit
AGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCT8300.11851978780674136No Hit
GGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCT7720.1102376821527763No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCA201.8388899E-593.984583
CGCTACG3400.087.0739443
CGTTTTT32800.086.2155761
ATAGCGG1150.081.830891
AGGGAAT13600.081.199915
TAGGGCA4650.080.846944
GTAGGGC3800.080.3815463
AGGGATG14450.079.675515
GTTACGG600.078.4212651
CGTAGGG1200.078.370842
AGGGCAC6450.077.95625
CGAAGGG6050.077.723152
GAAGGGC9450.077.07733
TAAGCGG1650.076.995421
GCTACGA3850.076.896474
AGGGCAT7850.076.624375
AAGGGCA10200.076.016944
AGTACGG1300.076.00831
CGAGGGA3350.075.748773
TAAGGGT3300.075.472463
Produced by FastQC (version 0.11.5)