FastQCFastQC Report
Mon 27 Feb 2023
SRR3128615.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128615.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences739126
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80291.0862829882861649No Hit
CCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTG43130.5835270305739482No Hit
GCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTG32680.4421438293335642No Hit
CTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC31930.43199670962731657TruSeq Adapter, Index 27 (95% over 22bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGGAGTTAC31600.42753197695656764No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC19820.2681545501037712No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA13580.18373051414779076No Hit
CGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCT12160.16451863417062856No Hit
AGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCT11030.14923030714654875No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATG10290.13921848236971773No Hit
ACTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTG9340.1263654640751374TruSeq Adapter, Index 27 (95% over 21bp)
AGAGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTT8290.11215949648639069No Hit
AAAGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTT8130.10999477761572453No Hit
GGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCT8090.10945359789805797No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG7530.10187708185072639No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTCG156.9020805E-493.997089
ACGGGTG850.088.467854
CGTTTTT36500.087.770831
AGGGCAT6550.085.3866655
ATAACGG500.084.677581
ACGGGTA800.082.247454
TACGGGT750.081.464143
GTACGGG2050.080.2577062
CGCTACG4000.079.897523
AGGGAAT13700.079.245735
AAGGGCA11000.079.043014
GCTACGA4050.078.911144
AGTACGG1550.078.9111
ATAGCGG1150.077.723381
AGAGGGC9150.077.560443
CGAGGGA4100.076.80253
AGGGTAT3800.076.681835
GACCGAT800.076.3726358
AGAGGGA18750.075.9496463
GGAATGC6500.075.920737
Produced by FastQC (version 0.11.5)