FastQCFastQC Report
Mon 27 Feb 2023
SRR3128614.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128614.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1179136
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114420.9703715262700825No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC58080.4925640468953539No Hit
CTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC55960.47458478072079896Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG46280.3924907729049067No Hit
GCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG43670.37035592162396874No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTCGCTAGT43150.36594591293964396No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA15940.1351837277464177No Hit
CGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCT15260.1294167933130699No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATG14850.12593967108119844No Hit
AGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCT14800.12551563178462874No Hit
AAAGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTT13950.11830696374294399No Hit
ACTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG13850.1174588851498046No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC13320.11296406860616588No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCTCG12950.10982617781155016No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGGG1500.090.900172
CGAAGGG8250.087.765682
CGAGGGA9400.084.9916843
CGCTACG10150.084.267623
CGTTTTT47850.083.988731
TCCGCTA10500.082.465751
GCGGGTA2000.082.241944
GCTACGA10350.082.185194
CGACCAA10750.080.438648
AGGGATG32600.080.007515
CTACGAC10700.079.4968955
TAGGGTA4200.079.44464
TAAGGGA15850.078.869253
GGATGGC20000.078.717297
TCGAAGG1500.078.432091
AACGGGA5600.078.045933
ATAGCGG1450.077.891181
AAGGGAT31600.077.780364
TAGGGCA6850.077.525254
CGGGAAT4500.077.281325
Produced by FastQC (version 0.11.5)