FastQCFastQC Report
Mon 27 Feb 2023
SRR3128613.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128613.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1143134
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105580.9236012575953476No Hit
CTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC59490.5204114303310023Illumina Single End Adapter 1 (95% over 21bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTCGCTAGT57340.501603486555382No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC55810.48821922889180097No Hit
CCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG52350.457951561234291No Hit
GCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG49110.4296084273584724No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATG20480.17915659931381625No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA19320.1690090575558071No Hit
CGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCT16290.1425029786534212No Hit
ACTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG16130.14110331772128204No Hit
AAAGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTT15560.13611702565053616No Hit
AGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCT15020.1313931700045664No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCTCG14080.12317016202824865No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCTCGCTAGTTC13780.12054579778048768No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC12870.11258522622894604No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT45050.086.313551
GTACGGG2600.084.9465562
CGGGAAT3600.084.849855
CGCTACG11750.083.585253
GCTACGA11800.083.2347264
AGGGATG29650.082.734395
TCCGCTA12050.082.3310551
AGTACGG1200.082.282281
CCGTCCA401.036824E-982.23553
GTAGCGG2250.081.498641
CGAAGGG8150.081.2985462
CGAGGGA8450.080.6366653
CGACCAA12100.080.005928
CTACGAC12350.079.908435
AATGCGG1600.079.343631
AGGGAAT25650.079.3306055
ACGGGTA950.079.147394
TAAGGGA12450.078.885693
ACGACCA12300.078.3229457
AGCGGGA8650.078.228973
Produced by FastQC (version 0.11.5)