FastQCFastQC Report
Mon 27 Feb 2023
SRR3128611.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128611.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2126166
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183680.863902442236401No Hit
GCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTG41380.19462262118762128No Hit
CTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC33980.15981818917243526No Hit
CCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTG33860.15925379297759443No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC22130.10408406493190091No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT22010.10351966873706005No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGAATCACG21510.10116801792522313No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT57700.084.717241
CGTAGGG5200.075.046572
GTAGGGC9400.073.9956363
ATAGGGC12550.071.525663
ATAGCGG3750.071.511461
TAGGGCA11500.070.700174
AAGGGTA13100.070.675224
TAAGGGA19100.070.6188663
GTACGGG4950.070.288232
ACGGGTA1950.069.893314
CTACGAC5400.069.625525
AAGGGAC22950.069.420744
AGGGATG28200.069.329245
AGTAGGG31700.068.5233842
AGGGTAC11000.067.5051045
AGTAAGG11800.067.38091
AGGGCAT18350.067.102315
ACGGGAT7550.066.605344
AGGGAAT25350.066.556235
AAGGGCG8900.066.53544
Produced by FastQC (version 0.11.5)