FastQCFastQC Report
Mon 27 Feb 2023
SRR3128610.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128610.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2034770
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168970.8304132653813454No Hit
GCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTG47130.23162323014394745No Hit
CCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTG37930.1864092747583265No Hit
CTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC37530.18444345061112558No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGAATCACG27330.13431493485750234No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC20630.10138738039188705No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT55100.083.476951
ACGGGTA2750.075.191434
CGTAGGG5900.074.870992
TAGGGCA8750.073.04314
TAAGGGA16450.072.561383
GTACGGG4250.069.6609342
AGGGATG24800.069.3549965
ATAGGGC12900.069.215223
CGAAGGG18300.068.821072
AGTAGGG32400.068.749732
AGGGCAT15450.068.43885
AGTACGG2850.067.657871
AAGGGTA14600.067.595034
AGGGAAT25850.067.446855
AGCAGGG40100.067.3846742
AAGGGCG8800.067.287794
GGAGCTA35100.067.076179
AGGGCGA9700.066.858355
AGGGACT27550.066.525995
TAGCGGG9750.066.5138552
Produced by FastQC (version 0.11.5)