FastQCFastQC Report
Mon 27 Feb 2023
SRR3128609.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128609.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2301206
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213200.926470728826537No Hit
GCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTG52620.22866270990080853No Hit
CCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTG44000.19120409037695887No Hit
CTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC41050.17838472522668547No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC24540.10663973586024023No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT71050.084.299421
CGTAGGG5700.073.3970952
AGTACGG3000.070.575111
AAGGGAC22650.070.5487754
TAAGGGA19550.069.475213
AGGGAAT27550.069.089525
TAGGGCA10750.069.0760354
AGGGCAT18350.068.640045
AGGGATG28500.068.6004945
AGTAAGG13350.068.372391
ATAGGGC13900.067.9610443
GGGCGAT10800.067.88596
AGGGTAA11450.067.726295
ATAGCGG4050.067.380351
GTAAGGG32100.067.069362
AAGGGCA34450.066.847574
AGAAGGG73950.066.807972
AGCAGGG43950.066.740492
AGACGGG14850.066.4747242
GGAGCTA37650.066.410219
Produced by FastQC (version 0.11.5)