FastQCFastQC Report
Mon 27 Feb 2023
SRR3128608.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128608.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1268845
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116330.9168180510621864No Hit
CCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG63310.49895771351110657No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTAAGTCCT57730.4549807108039201No Hit
GCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG56130.44237081755454766No Hit
CTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC55420.43677517742513855No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC42660.33621127876139323No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA20510.16164306909039322No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATG19410.15297376748144967No Hit
CGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCT18330.14446208953812326No Hit
AGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCT18060.14233417005229165No Hit
ACTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG17960.14154605172420587No Hit
AAAGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTT17740.13981219140241716No Hit
AGAGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTT14890.11735081905197245No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC14620.11522289956614085No Hit
GGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCT13710.10805102278056028No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT49600.088.902451
CGTAGGG3000.087.762992
GCGGGAT7650.084.158584
CGGGATT5050.082.8204965
CGAGGGA8200.082.5254443
CGAAGGG10250.082.10582
AGGGAAT27800.081.985335
ACGGGTA1150.081.7280964
AGGGATG31300.081.675875
TAGACGG1650.079.860111
AGCGGGA9700.079.4531863
TCCGCTA7800.079.037371
AAGGGAT33000.078.749974
GGATGGC20650.078.7399757
GCTACGA7950.078.027214
GGGAATG29600.077.952316
TAACGGG3200.077.870062
AGGGATT22700.077.4256745
TAAGGGA16800.076.924143
AATCGGG1900.076.710142
Produced by FastQC (version 0.11.5)