FastQCFastQC Report
Mon 27 Feb 2023
SRR3128607.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128607.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1230385
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107670.8750919427658823No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTAAGTCCT74010.6015190367242773No Hit
CCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG70220.5707156702983213No Hit
CTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC65460.5320285926762761No Hit
GCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG64550.5246325337191204No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC39340.31973731799396127No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATG25470.20700837542720366No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA24400.1983119104995591No Hit
ACTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG20840.1693778776561808No Hit
AGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCT20640.16775237019306966No Hit
AAAGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTT19330.15710529630969167No Hit
CGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCT19290.15678019481706945No Hit
AGAGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTT16880.13719282988658021No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCTAAGTCCTTC16020.1302031477952023No Hit
GGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCT14900.12110030600177993No Hit
ACCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCT14160.1150859283882687No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC12910.10492650674382409No Hit
TCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG12600.10240697017600182No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA950.093.99524
CGTAGGG2600.088.572412
CGTTTTT46000.087.509751
GCGGGAT7700.087.281264
AGGGAAT28000.083.417355
AGGGATG26350.081.8634645
GCTACGA7600.081.627414
CGAGGGA7950.080.989583
CGGGATT4200.080.564045
TAAGGGA15600.080.438213
CGGGTAT1000.079.892685
TCCGCTA7900.079.7660751
TAGGGCA5050.079.104884
GGGAATG30250.078.921696
AGCGGGA9450.078.5783
GGAATGC14000.077.878577
ATAACGG850.077.455031
GATGACC8900.077.0940558
TAACGGG2750.076.905172
GGATGGC17000.076.575337
Produced by FastQC (version 0.11.5)