FastQCFastQC Report
Mon 27 Feb 2023
SRR3128606.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128606.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1347682
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138201.025464464168847No Hit
CCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG79540.5901985780028226No Hit
CTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC74380.5519106139282115No Hit
GCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG68230.5062767032578902No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC44440.32975138051854963No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTAAGTCCT39740.2948766845591171No Hit
AAAGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTT22980.17051500279739584No Hit
AGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCT22850.16955038354745408No Hit
CGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCT21310.15812335550968254No Hit
ACTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG20650.15322605777920903No Hit
AGAGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTT17950.1331916579727265No Hit
GGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCT15950.11835136181977647No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC15900.11798035441595273No Hit
ACCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCT14940.1108570122625367No Hit
TCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG14850.11018919893565396No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA14110.10469828935906246No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCGG353.5652192E-1094.070731
CGTTTTT52850.087.662891
ACGGGTA1350.087.034664
CGTAGGG3600.086.167522
ATAGCGG2400.084.27171
TAGGGCG950.084.1029744
AGTACGG2000.082.31191
CGAGGGA7850.082.023253
AGGGATG30350.081.9186865
CGAAGGG10150.081.4983442
AGGGAAT31450.080.996215
GCTACGA9000.080.942244
TAAGGGA18950.080.108643
TCCGCTA9250.079.8331
AGCGGGA10650.079.4344563
ATAACGG1250.079.019421
GCGGGAT7650.078.638384
TAACGGG3000.078.334112
AAGGGAT37450.077.306314
CTACGAC9450.077.0878455
Produced by FastQC (version 0.11.5)