FastQCFastQC Report
Mon 27 Feb 2023
SRR3128605.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128605.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2586844
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217020.8389373305850681No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC72800.2814240054676664No Hit
CCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTG36650.1416784313240381No Hit
GCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTG35620.13769674553239392No Hit
CTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC33440.1292694882258072Illumina Single End Adapter 2 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT27710.10711894493831092No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT74100.082.627861
AGGGATG51050.073.829995
AGTAGGG42950.073.345892
CGTAGGG6450.072.896122
TAGGGAT21200.071.1579364
TAAGAGG16250.070.954531
ATAGGGA29450.070.692383
CTACGAC15800.070.492915
TACGACC15950.070.124616
AGGGAAT33950.070.043035
TAGGGCA15200.069.874554
TAGCGGG13400.069.825232
TAGGGTA10100.069.329664
ACGGGTA2850.069.2561954
GTAGGGC12550.068.901443
CGCTACG16100.068.887483
ACGACCA16050.068.516487
GAGGGAT36850.068.48434
TAAGGGA25900.068.224793
GTACGGG6550.068.194042
Produced by FastQC (version 0.11.5)