FastQCFastQC Report
Mon 27 Feb 2023
SRR3128604.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128604.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2434404
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200270.8226654244735057No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC66850.2746052011087724No Hit
CCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTG41680.17121233780424283No Hit
GCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTG41060.16866551320158857No Hit
CTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC38590.15851929260714326Illumina Single End Adapter 2 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT25070.10298208514281113No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCACCATAGA24410.10027094927546948No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT64000.083.541711
AGGGATG47650.075.9437265
CGTAGGG6150.075.651092
TAGGGCA12750.074.822484
GAGGGAT36050.073.261744
TAAGGGA23700.072.3768543
TAGGGTA10050.072.0113454
AGGGAAT31550.071.4999855
ATAGGGA26650.071.418873
GTAGGGC10700.070.712673
GAATAGG17400.070.536541
GGTAAGG13900.069.690771
TAACGGG8450.069.519792
AAGGGAC25400.069.381514
AGTAGGG39850.069.225312
CGAAGGG25500.069.110852
GGATGGC36600.068.564217
AGATAGG16300.068.372831
TAGAGGG43750.068.3178562
AGGGTAA14450.068.299195
Produced by FastQC (version 0.11.5)