FastQCFastQC Report
Mon 27 Feb 2023
SRR3128603.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128603.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2726374
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT248410.9111369166519341No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC77700.28499391499478793No Hit
CCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTG47590.1745541880901153No Hit
GCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTG46410.17022609517256254No Hit
CTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC44800.16432081585285072Illumina Single End Adapter 2 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT28120.10314065495049468No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT78450.083.373711
TAGGGCA15100.075.320244
AGGGATG54100.074.62245
CGTAGGG6700.072.256262
AGGGAAT34800.072.2516565
CTACGAC15250.072.113955
TAACGGG8950.071.946662
TAAGGGA26750.071.506233
ATAGGGA27350.070.796733
AGATAGG16250.070.0761
ATAGGGC18200.069.721333
GTACGGG7150.069.680772
AGAGGGA65350.069.327373
AGTAGGG41650.069.2893452
AGGGCAT22900.069.1620255
AGGGACT34850.068.7765965
GTAGGGC13600.068.7681663
TAGGGAT22350.067.92014
GAGGGAT39400.067.8717964
GGTAAGG17750.067.865471
Produced by FastQC (version 0.11.5)