FastQCFastQC Report
Mon 27 Feb 2023
SRR3128602.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128602.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3078607
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230200.7477407801645355No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC71120.23101357204735778No Hit
CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG42190.1370425000657765No Hit
GCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG42060.13662023116299027No Hit
CTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC40030.1300263398348669Illumina Single End Adapter 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT92300.079.725751
ATAGGGC16100.075.018743
ACGACCA16250.069.988157
AGGGATG54200.069.9740145
GAATAGG19050.069.896441
AGTAGGG50650.068.599032
ATAGGGA35150.068.4553153
TAAGGGA31650.068.452573
TACGACC17050.068.358086
CGAAGGG31800.068.307422
CTACGAC17050.068.0824365
CGAGGGA21750.067.631283
AAGGGAT65750.067.617314
TAGGGTA10050.066.870174
TAGCGGG14550.066.566732
TAGGGCA15150.066.384034
GAGGGAT53950.066.2911764
CGCTACG17600.065.954863
AAGAGGG102150.065.910962
AGGGATC30100.065.732195
Produced by FastQC (version 0.11.5)