FastQCFastQC Report
Mon 27 Feb 2023
SRR3128601.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128601.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2848490
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210220.7380050482887425No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC64480.2263655480623067No Hit
CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG46600.16359544881674148No Hit
GCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG46460.1631039603439015No Hit
CTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC45500.15973375367299866Illumina Single End Adapter 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT82500.079.394841
AGGGATG49300.072.257175
ACGGGTA3300.071.2044454
GAATAGG17200.070.8054051
CGAAGGG27650.070.703622
TAGCGGG11450.069.772922
ATAGGGA32750.069.4508363
CTACGAC13550.069.366445
CGAGGGA17900.069.309753
AGTAGGG47100.069.243972
GCTACGA13650.069.201334
TACGACC13500.068.92596
TAGGGCA12700.068.827234
TAAGGGA29550.068.702053
AAGGGAT60300.068.349194
CGCTACG13900.068.29363
AGGGAAT35150.067.6525045
TAGGGCG4100.067.6268544
ATAGGGC15500.067.610883
ACGACCA13600.067.382457
Produced by FastQC (version 0.11.5)