FastQCFastQC Report
Mon 27 Feb 2023
SRR3128600.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128600.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3203125
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT264290.8251004878048781No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC76510.23886048780487804No Hit
CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG54240.16933463414634148No Hit
CTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC52690.16449560975609756Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG50790.1585639024390244No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT99100.079.6467741
AGGGATG58000.071.874285
ATAGGGA34750.071.1391753
CGAAGGG32700.069.293492
AGGGAAT39200.069.177945
AGTAGGG52650.068.841312
ATAGCGG5150.068.542961
ATAGGGC17950.068.074873
AAGGGAT72750.068.025744
GAATAGG17300.068.014691
GTAGGGA30200.067.539943
ACGGGTA4400.067.292364
TAAGGGA35200.067.025333
CGAGGGA23500.066.596943
TAGAGGG56450.066.5389562
TATAGGG26450.066.472052
TAGGGCA15500.066.40344
GCTACGA16000.066.0907064
TACGACC16100.065.680216
AGAGGGA85800.065.457363
Produced by FastQC (version 0.11.5)