FastQCFastQC Report
Mon 27 Feb 2023
SRR3128599.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128599.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2678980
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216850.8094498652472211No Hit
GCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTG29830.11134834899849944No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT27200.10153117977737797No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT77350.079.400681
CGTAGGG7850.077.2703252
GTACGGG6650.073.536742
TAGGGCG3850.072.020644
ATAGCGG6150.071.932771
GTAGGGC12150.071.558493
TAGGGCA15850.069.975894
AGGGATG43650.068.691365
AGGGTAC13500.068.580115
GCATAGG7850.068.345391
ATAGGGC16600.068.229893
AGTAGGG42250.067.8884352
ATAGGGA26900.067.4373863
ACGGGTA3300.066.934434
AGATAGG14750.066.685021
AATAGGG38550.066.4762
TATAGGG18150.066.062692
GTAGGGA25500.065.979423
TAGGGAT21600.065.9256744
ATGCGGG13200.065.900772
Produced by FastQC (version 0.11.5)