FastQCFastQC Report
Mon 27 Feb 2023
SRR3128598.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128598.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2541525
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199550.7851585170320968No Hit
GCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTG34440.13550919231563727No Hit
CCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTG30660.12063623218343318No Hit
CTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC28460.11198001200066889Illumina Single End Adapter 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT71500.081.4018251
CGTAGGG6900.074.239422
TAGGGCG3750.073.9411854
GTAGGGC11450.073.0586553
ATAGCGG5800.072.140891
ATAACGG2000.070.519751
AGTAGGG38900.070.191362
AGGGAAT28200.069.994815
GAATAGG14600.069.231721
ATAGGGA23950.069.070773
GGGCGAT12050.068.639516
AGATAGG13700.068.632361
ATGCGGG13000.067.962862
GTAGGGA22700.066.870383
TAGGGCA12600.066.391914
AGGGATG39200.066.178775
AGGGCAT22350.066.026435
AAGGGTA18400.065.897294
AAGGGCG10600.065.617784
TGTAGGG19750.065.43692
Produced by FastQC (version 0.11.5)