FastQCFastQC Report
Mon 27 Feb 2023
SRR3128597.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128597.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2850289
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT248020.8701573770238737No Hit
GCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTG38900.1364773887840847No Hit
CCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTG34580.12132103095510666No Hit
CTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC31980.1121991489284069Illumina Single End Adapter 2 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT29550.10367369764960675No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT81150.082.338341
CGTAGGG7850.073.6487052
GTAGGGC12550.073.024263
GTACGGG6300.070.87822
AGGGAAT32050.070.826445
TAGGGCA15700.070.3468254
ATAGGGC16950.068.4862753
AGTAGGG44250.067.769932
ATAGGGA26700.067.7681663
AGGGTAC12650.067.6171955
GAATAGG18050.066.997441
TAGGGAT20450.066.876884
ATAGCGG6900.066.831211
TGTAGGG19000.066.7943652
TAGAGGG46200.066.639082
ATGCGGG13550.066.602662
TAAGGGA25900.066.5951463
AATAGGG39850.066.524312
AGGGATG44400.065.733225
TATAGGG21350.065.6066742
Produced by FastQC (version 0.11.5)