FastQCFastQC Report
Mon 27 Feb 2023
SRR3128596.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128596.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2640138
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202950.768709817441361No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC40630.15389347072009113No Hit
GCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTG35880.13590198694159167No Hit
CTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC33020.1250692198665373No Hit
CCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTG29540.11188809069828926No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT74650.081.0191041
TAGGGCA12150.074.651184
AGGGATG42500.073.2022865
TAAGGGA23850.072.5128563
ACGGGTA4100.071.066254
CGAAGGG28000.070.870432
ATAAGGG35050.070.568092
CGTAGGG8300.070.251282
ATAGGGC13750.069.382333
ATAGGGA28550.069.1351243
AGGGAAT33600.068.6748665
AGTAAGG16150.068.4872061
CGGGTAT3100.068.219175
ATGCGGG12000.067.7915342
AGTAGGG41700.067.7718052
GAATAGG17400.067.624621
AGGGCAT23700.067.2213065
AAGGGTA20650.067.136334
AGGGTAC13450.067.086415
GTAAGGG37550.066.7464142
Produced by FastQC (version 0.11.5)