FastQCFastQC Report
Mon 27 Feb 2023
SRR3128595.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128595.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2534053
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192980.7615468184761723No Hit
GCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTG41970.16562400233933544No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC39780.1569817205875331No Hit
CTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC37700.1487735260470085No Hit
CCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTG32110.12671400321934861No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT68800.081.6026151
TAGGGCA13600.075.675164
ACGGGTA3800.074.2020344
CGTAGGG9200.073.555352
ATAGGGC15350.072.5570453
AGGGATG41400.072.197495
AGGGTAC11250.071.434655
TAGGGTA9450.070.616264
TAAGGGA22000.068.995293
CGAAGGG24300.068.4599462
AGTAGGG41350.068.416462
ATAAGGG31900.068.354472
ACGGGAT9600.068.04434
AAGGGTA19650.067.920984
GAATAGG17500.067.709661
AGGGAAT32650.067.65195
AGGGCAT22950.067.576645
AAGGGAT49950.067.081414
AAGAGGG81550.066.5575942
AGAGGGC35250.066.524413
Produced by FastQC (version 0.11.5)